Effect of Brassica Oleracea Biscuit Supplementation on Immunity and Hemoglobin Levels in Pregnant Women: A Quasi-Experimental Study

Authors

  • Febriana Sari Mitra Husada Medan College of Health Sciences, Medan, North Sumatra, Indonesia
  • Rolasnih Lilista Simbolon Mitra Husada Medan College of Health Sciences, Medan, North Sumatra, Indonesia
  • Edi Subroto Mitra Husada Medan College of Health Sciences, Medan, North Sumatra, Indonesia
  • Trisnawati Trisnawati Kampung Baru Community Health Center, Medan, North Sumatra, Indonesia
  • Jemalemna Sembiring Bangun Rejo Village Sub-Health Center, North Sumatra, Indonesia
  • Oca Floren Simanullang Mitra Husada Medan College of Health Sciences, Medan, North Sumatra, Indonesia
  • Kartika Ovalia Ginting Mitra Husada Medan College of Health Sciences, Medan, North Sumatra, Indonesia

DOI:

https://doi.org/10.55018/janh.v8i1.561

Keywords:

Pregnancy, Anemia, Iron-Deficiency, Brassica, Functional Food, Hemoglobins, Immune System

Abstract

Background: Maternal mortality remains a major concern, with anemia as a key contributing factor during pregnancy. Limited adherence to iron supplementation highlights the need for alternative approaches. Brassica oleracea, rich in essential nutrients, may support immune function and hemoglobin synthesis. This study aimed to evaluate the effect of this product in biscuit form on immunity and hemoglobin levels in pregnant women.

Methods: A quasi-experimental study with a pre-test–post-test control group design was conducted. Baseline measurements were obtained from both intervention and control groups. The intervention group received Brassica oleracea biscuits in addition to standard care, while the control group received standard care alone. Post-intervention assessments were conducted in both groups.

Results: There was a statistically significant difference in immune status between the intervention and control groups (p = 0.044). However, no significant difference in hemoglobin levels was observed between groups (p = 0.560).

Conclusion: Brassica oleracea biscuit supplementation significantly improved immune status in pregnant women but did not result in a statistically significant difference in hemoglobin levels compared to standard care. These findings suggest that Brassica oleracea–based functional foods may serve as a complementary strategy to enhance maternal immunity during pregnancy.

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References

Anam, S., Nashihin, H., Taufik, A., Sitompul, H. S., Manik, Y. M., Sugiyono, Jayali, A. M., & Sriwahyuni, E. (2023). Metode penelitian (kualitatif, kuantitatif, eksperimen, dan R&D). Journal on Teacher Education, 4(2).

Dinas Kesehatan Kabupaten Deli Serdang. (n.d.). Profil kesehatan Kabupaten Deli Serdang tahun 2023.

Gibran, P. (2024). Bersama Indonesia maju.

Hasanah. (n.d.). Hubungan tingkat kepatuhan konsumsi tablet tambah darah dengan kejadian anemia pada ibu hamil trimester III di Puskesmas Pujer.

Henny, S. (2021). Metodologi penelitian kesehatan. Ahlimedia Press.

Husaeni, N. (2023). Pengaruh pemberian kombinasi brokoli dan alpukat terhadap kadar hemoglobin pada ibu hamil di Kabupaten Bogor.

Indonesia, Kementerian Kesehatan Republik Indonesia. (n.d.-a). Peraturan Menteri Kesehatan Nomor 88 Tahun 2014 tentang pemberian tablet tambah darah (TTD) untuk ibu hamil.

Indonesia, Kementerian Kesehatan Republik Indonesia. (n.d.-b). Survei Kesehatan Indonesia Tahun 2023.

Listiana, A., & Jasa, N. E. (2023). The relationship between age and parity with the incidence of chronic energy deficiency (KEK) in pregnant women. Jurnal Midpro, 14(2), 195–202. https://doi.org/10.30736/md.v14i2.454

M. Risya Rizki, S.KM., M. K., & Sri Nawangwulan, SKM, M. K. (2018). Metodologi penelitian kesehatan. Rake Sarasin.

Nisak, K. (2024). Faktor-faktor yang mempengaruhi pengetahuan ibu hamil tentang makanan yang mengandung zat besi di Puskesmas Peureulak Aceh Timur.

Nurhasanah, I. (2022). Analisis kandungan vitamin C dan zat besi (Fe) pada brokoli (Brassica oleracea).

Organization, W. H. (n.d.). Climate inaction is claiming millions of lives.

Peraturan Menteri Kesehatan Republik Indonesia Nomor 6 Tahun 2024 tentang standar teknis pemenuhan standar pelayanan minimal (SPM). (n.d.).

Rahayu, A. (2025). Metode penelitian. Diajar, 4(3), 459–470. https://doi.org/10.54259/diajar.v4i3.5092

Sinaga, R., Dewi, E. R., Pinem, S., Purnamasari, E., & Sagala, R. (2024). The effect of Beta vulgaris L. juice on the reduction of anemia in pregnant women. 12(5).

Syeffryna, B. (2025). Pengaruh pemberian puding brokoli (Brassica oleracea var. italica) terhadap kadar hemoglobin pada ibu hamil anemia di wilayah kerja Puskesmas IV Koto Kabupaten Agam tahun 2025.

Utara, Dinas Kesehatan Provinsi Sumatera. (2023). Profil kesehatan Provinsi Sumatera Utara tahun 2023.

WHO. (2024). Guideline on haemoglobin cutoffs to define anaemia in individuals and populations.

Wibowo, N., Rima, I., & Rabbania, H. (2021). Anemia defisiensi besi pada kehamilan. https://www.pogi.or.id

Yulizawati, M. K. H. (2024). Buku ajar asuhan kebidanan pada kehamilan.

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Published

2026-03-21

How to Cite

Sari, F. ., Simbolon, R. L. ., Subroto, E. ., Trisnawati, T., Sembiring, J. ., Simanullang, O. F. ., & Ginting, K. O. . (2026). Effect of Brassica Oleracea Biscuit Supplementation on Immunity and Hemoglobin Levels in Pregnant Women: A Quasi-Experimental Study. Journal of Applied Nursing and Health, 8(1), 624–635. https://doi.org/10.55018/janh.v8i1.561

Issue

Vol. 8 No. 1 (2026): Journal of Applied Nursing and Health

Section

Articles

License

Copyright (c) 2026 Febriana Sari, Rolasnih Lilista Simbolon, Edi Subroto, Trisnawati Trisnawati, Jemalemna Sembiring, Oca Floren Simanullang, Kartika Ovalia Ginting

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